Allele-Specific Recombinase Polymerase Amplification to Detect Sickle Cell Disease in Low-Resource Settings
نویسندگان
چکیده
منابع مشابه
Haptoglobin genotyping by allele-specific polymerase chain reaction amplification.
We performed haptoglobin (Hp) genotyping by polymerase chain reaction using allele-specific primer-pairs. The major six genotypes of Hp were identified using this method. Among Japanese individuals living in Ehime and Okayama Prefectures, the allele frequencies were estimated to be Hp2 = 0.723 and Hp1s = 0.277. Genotyping of Hp was possible with 0.3 ng of DNA and with 0.125 microliter of blood....
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ژورنال
عنوان ژورنال: Analytical Chemistry
سال: 2021
ISSN: 0003-2700,1520-6882
DOI: 10.1021/acs.analchem.0c04191